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Animal
Mouse, Rat
Dynamic Plantar Aesthesiometer - For Automated Mechanical Stimulation and Allodynia
Product Code:37550-
Category: Pain and Inflammation
Touch sensitivity is traditionally tested with Von Frey filaments, which is a tedious and experimenter-dependent assay.
The Dynamic Plantar Aesthesiometer is a unique system, which fully automates the amount of force applied by an electromagnetic, silent motor exerting from 0 to 100 grams at a rate adjustable from 0 to 50 seconds.
When the animal responds, by withdrawing its paw, the device automatically scores the time and the force at which the response occurred and the data can be stored or exported in CSV format.
One model for Mice or Rats in modular enclosures (up to 6 rats or 12 mice)
Background
- Von Frey filaments are still a productive way to evaluate behavioral responses of neuropathic pain in preclinical and clinical research.
- However, Von Frey tests are time consuming and subject to experimenter bias, and for this reason Ugo Basile developed a fully automated instrument
- Moreover, the application of the force is always perpendicular, with a preset amount of grams and at a predefined rate
- The Dynamic Plantar Aesthesiometer has been used in a variety of applications in nerve injury, from partial sciatic nerve ligation (PNL, to chronic constriction injury (CCI) and spinal nerve ligation (SNL) to screen phenotypes and drugs to treat allodynia and hyperalgesia
System components and main features
The Ugo Basile system comes complete with:
- A movable touch stimulator with standardized filament and angled mirror to facilitate paw targeting
- A touch-screen based electronic unit, which controls all the setting parameters, collects data and saves into USB stick for seamless export into CSV, Excel-compatible format
- A large testing grid surface with 5x5 mm square holes
- A modular set of animal enclosures to hold up to 6 rats and 12 mice
Additional features include:
- TTL outputs for start and stop
- Automatic or manual detection of paw withdrawal
- Analogue output is also available
- Filament approaching curve can be adjusted to provide a smoother or lighter first contact with the animal paw, before the force application ramp starts
| Feature | Benefit |
|---|---|
|
Fully automated force and force rate application |
Higher throughput and improved accuracy |
|
Automated scoring of paw withdrawal |
Higher throughput and improved accuracy, thanks to decrease in experimenter bias |
|
Large force range (0-100 grams) |
Flexibility for performing many types of experiments from severe allodynia to hyperalgesia |
|
Touch force and touch pause can be adjusted |
The way the probe approaches the paw can be changed, depending on paw sensitivity |
|
Touch screen and multiple data output (internal storage and CSV export) |
Data can be quickly saved in the electronic unit or on the USB stick for CSV export and further analysis |
|
Optional manual scoring of paw withdrawal |
Allows for scoring animals which present an unusual position of the paw, and do require the experimenter to visually score |
|
Painted metal grid and modular animal cages |
Works with rats and mice in high throughput (6 rats and 12 mice at the time) |
| General | |
|---|---|
| Read out and commands |
4,3 inches touch-screen (Resistive) |
| Power Requirements | Universal input 100-240 VAC, 50-60 Hz, 30W |
| Sound Level | Negligible |
| Operating Temperature |
10°C to 40°C; 5% to 95% RH (non-condensing) |
|
Operation | |
| Force Range |
0.1 to 100 grams, in 0.1g steps |
| Force Increasing Rate (ramp) | Adjustable in the interval 0 to 50 seconds, in 1 s steps |
| Latency Time | Display in 0.1s steps |
|
Measurement Mode |
Automatic |
|
Start Experiment |
By Start button, push buttons or TTL input |
| Stop experiment |
By Stop button, push buttons, cut-off or TTL input |
| Data input | Experiment protocol |
|
Data export |
.csv format |
| TTL/IO | Start/Stop, Detection Mode, Force level |
| Calibration | Calibration Weights (5g, 50g, 100g) |
|
Physical | |
| Total Weight | 10Kg |
| Shipping Weight | 13Kg |
| Dimensions (all parts) | 135 (w) x 40 (d) x 50 (h) cm (required space on table; all parts) |
| Packing Dimensions | 98x49x47 cm |
|
Warranty | |
| Warranty | Dynamic Plantar Aesthesiometer is covered by a 12-month warranty + 12 after product registration. |
| UB-Care | Additional UB-Care can be added for other 12 or 24 months |
The Dynamic Plantar Aesthesiometer has been used in a variety of applications in nerve injury, from partial sciatic nerve ligation (PNL, to chronic constrinction injury (CCI) and spinal nerve ligation (SNL) to screen phenotypes and drugs to treat allodynia and hyperalgesia.
The main advantages of the Dynamic Plantar Aesthesiometer over the Von Frey Hairs, Semmes-Weinstein set of monofilaments and the Electronic Von Frey systems (hand-held) are:
- Consistency in the vector of the force application (perpendicular)
- Easier reproducibility of the site of stimulation
- Removal of experimenter bias in paw withdrawal scoring and addition of the time component, being the force rate adjustable
Altogether this brings about a high reproducibility, sensitivity and accuracy which no other mechanical stimulation application can provide. The sensitivity is by far the result of the fact that the force application does not rely on hand movement.
Moreover, the Dynamic Plantar Aesthesiometer, differently than Von Frey filaments, provide a linear and not logarithmic scale of stimulation.
| 37550 Dynamic Plantar Aesthesiometer | |
|---|---|
| 37550-001 | Microprocessor controlled electronic unit |
| 37550-002 | Touch Stimulator, complete with probe actuator and adjustable angled-mirror |
| 37000-003 | Large Platform |
| 37400-327 | Supporting column (4) |
| 37000-007 | Modular Animal Enclosure |
| 37450-005 | Large Framed Perforated Metal Sheet |
| 37450-321 |
Plastic Case lodging, 2 Filaments (with umbrella), 2 Calibration Weights, 1 hex. Wrench 1.25 |
| E-AU 101 | Memory Key, including the user manual and Xpad software |
| Optional | |
| 37450-278 | Additional Stimulation Base, complete with perforated metal sheet and animal enclosure |
| 37100 | Set of 2 Durham Holders for Orofacial Stimulation |
| 37102 | Rat Holder, medium size, for use with Plantar Test and Dynamic Plantar Aesthesiometer |
| 37103 | Rat Holder, large size, for use with Plantar Test and Dynamic Plantar Aesthesiometer |
Claudia Cristiano et al., 2022, The Beneficial Effects of Ultramicronized Palmitoylethanolamide in the Management of Neuropathic Pain and Associated Mood Disorders Induced by Paclit*xel in Mice, Biomolecules
Hisakatsu Ito et al., 2022, Suvor*xant and mirtaz*pine improve chronic pain-related changes in parameters of sleep and voluntary physical performance in mice with sciatic nerve ligation, Plos One
Katherine S. Adcock et al., 2022, Vagus nerve stimulation does not improve recovery of forelimb motor or somatosensory function in a model of neuropathic pain, Nature
Laura Rullo et al., 2021, Nociceptive behavior and central neuropeptidergic dysregulations in male and female mice of a Fabry disease animal model, Elsevier
Huiying Du et al., 2019, Identification of the Genome-wide Expression Patterns of Long Non-coding RNAs and mRNAs in Mice with Streptozotocin-induced Diabetic Neuropathic Pain, Elsevier
Miodrag Mitrić et al., 2019, Layer- and subregion-specific electrophysiological and morphological changes of the medial prefrontal cortex in a mouse model of neuropathic pain, Nature
Viljami Jokinen et al., 2018, Differential Spinal and Supraspinal Activation of Glia in a Rat Model of M*rphine Tolerance, Elsevier