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Animal
Mouse, Rat
Videotracking
Traditional
Fear Conditioning System
Product Code:46000
Category: Behaviour, Conditioning, Reward
Fear conditioning is one of the most common methods to measure memory in rodents. It exploits the Pavlovian association between an unpleasant unconditioned stimulus (shock) to a conditioned neutral stimulus (sound cue). The reaction of rodents, as predated animals, is to freeze (stay immobile) when exposed to fearful situations, such as a shock.
Once the association has happened and the animal has memorized it, freezing will happen also when the sound is presented without shock. This is the principle of Fear Conditioning, which is also coupled to the change of the change context (appearance) to ensure that the animal is reacting to the association to the sound and not to the environment (cued and contextual fear conditioning)
Background
- Fear conditioning is one of the most common methods to measure memory in rodents
- It exploits the Pavlovian association between an unpleasant unconditioned stimulus (shock) to a conditioned neutral stimulus (sound cue or context)
- The reaction of rodents, as predated animals, is to freeze (stay immobile) when exposed to fearful situations, such as a shock
- The ratio between the freezing with and without shock gives a measure of the memorization of the event and hence allows for a fast memory screening
Easy control, easy set up, easy expansion
The Ugo Basile system comes complete with:
- Visible and IR lights
- Ventilated isolation cubicle
- Shocker and electrified cage
- High intensity speaker for frequency and white noise sounds
- IR camera, cables and lenses
- Color-coded easy connections with the onboard electronics
- Seamless to be expanded from 1 to 4 or more units for high throughput
- Detection of the Freezing is automated through Any-maze or any other software, as well as the control of lights, sounds, shocker, etc.
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Features |
Benefits |
|---|---|
|
One USB cable connection |
Seamless installation and connection to PC |
|
Miniaturized USB-IR camera with 2 lenses (normal and fish-eye) |
Camera mounted on the ceiling and working in any light conditioning and with any cage dimension |
|
Interchangeable contexts |
Easy to mount on the walls with different patterns |
| IR and visible illumination | Experiments can be run in light or darkness, also as a tool to alter the context |
| Same software controls devices and analyses freezing | Fast learning curve in building protocols and automatic detection of freezing |
| Version for tethered animals | Compatible with optogenetics and electrophysiology |
| Multiple cage dimensions | Most suitable dimension for rats or mice |
| Operation | ||
|---|---|---|
| Camera | IR (lens 2.1mm and 4.3mm) | |
| Active Silent Ventilation | Yes | |
| Read-Out | USB to PC, TTL I/O | |
|
General | ||
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Shock |
Constant current |
|
|
Shock Intensity |
from 0.1 to 2.9mA, in 0.1mA steps |
|
|
Shock Duration |
Duration: from 0.5 to 99 seconds |
|
|
Illumination |
Dimmable visible and IR |
|
|
Sound |
500Hz-18KHz, 100dB ± 3dB |
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| White Noise | 77dB ± 3dB | |
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Power |
100-240 VAC, 50/60 Hz |
|
|
Operating Temperature |
10°C to 40°C; 5% to 95% RH (non-condensing) |
|
|
Sound Level |
Negligible (sound cue excluded) |
|
|
Pollution Degree |
≤ 2 |
|
|
Physical | ||
| Dimension | Outer Size | Internal Size |
|
Cubicle |
55(d)x60(w)x57(h)cm |
39(d)x47(w)x48(h)cm |
| Mouse Cage | 24(w)x20(d)x30(h)cm | 17x17x25(h)cm |
| Rat Cage | 33(w)x29(d)x41(h)cm | 25.5x25.5x36(h)cm |
| Mouse Cage XL | 33(w)x29(d)x41(h)cm | 25.5x25.5x36(h)cm |
| Observational Mouse | 34(w)x17(d)x30(h)cm | |
|
Weight |
30Kg |
|
|
Shipping Weight |
40Kg |
|
|
Warranty | ||
| Warranty | Fear Conditioning is covered by a 12-month warranty + 12 after product registration | |
| UB-Care | Additional UB-Care can be added for other 12 or 24 months | |
| PC Requirements | |||
|---|---|---|---|
| PC Requirements |
Minimum specification |
Recommended specification |
Best system |
|
Windows version |
XP service pack 3 |
Windows 10 |
Windows 10 |
|
Processor |
Pentium Dual-core |
Core i3 |
Core i7 |
|
RAM |
1GB |
4GB |
8GB |
|
Hard drive |
1GB available |
500GB |
1TB |
|
Display |
1366 x 768px |
1600 x 900px |
1920 x 1080px |
|
Expansion |
Not applicable |
Not applicable |
PCi express ports |
If you're planning to track in multiple apparatus, or track for long periods (> 12 hours), or include large numbers (> 500) of animals in experiments then we recommend you read the whitepaper Choosing a computer for ANY-maze which discusses various technical considerations that you may need to take into
Fear conditioning is one of the most common methods to measure memory in rodents. It exploits the Pavlovian association between an unpleasant unconditioned stimulus (shock) to a conditioned neutral stimulus (sound cue or context). The reaction of rodents, as predated animals, is to freeze (stay immobile) when exposed to fearful situations, such as a shock, or to a neutral stimulus they have associated (i.e. they remember) to it.
Once the association has happened and the animal has memorized it, freezing will happen also when the sound is presented without shock or when the animal experiences the context where the shock was delivered.
This is the basic principle of Fear Conditioned, which is normally referred to as Cued and Contextual to describe change in the cage interior (context, or appearance). This step of the experiment ensures that the detected freezing is actually the reaction to the sound and not to the memory of the environment. In the same way, the memorization of the context as the unconditioned stimulus (with no sound used), can be measured by comparing the freezing with shock (training), or in the same context without shock.
One of the reasons of the success of Fear Conditioning in memory studies is indeed its simplicity and the fact that it can be frequently used as a single trial, fast and high-throughput experiment.
|
Fear Conditioning System | |
|---|---|
|
46001 |
Fear-Conditioning Unit, including Isolation Cubicle 46000-596 (inside dimensions 48.5(w)x38.5(d)x48.5(h)cm), dual visible/I.R. light 46000-325, speaker 46000-165, controller on-board 46000-105, camera 47400-035, cables and power supply. System to be completed with software ANY-maze (full license or FC-version) and mouse or rat cage |
|
46001-2 |
Bundle of 2 FC-Units |
|
46001-3 |
Bundle of 3 FC-Units |
|
46001-4 |
Bundle of 4 FC-Units |
|
Fear Conditioning - Cages | |
|
46002 |
Rat Cage, I.D. 26x26x30(h)cm |
| 46002-D03 | Mouse XL Cage, I.D. 26x26x30(h)cm, with mouse floor |
|
46003 |
Mouse Cage, I.D. 17x17x25(h)cm |
| 46003-D03 | Mouse Cage with Front Door |
| 46004-D03 | Mouse Cage XL for cabled animals |
| 46005 | Fear Conditioning Cage for Observational Learning, I.D.34x30(h)cm; (17x17 cm for each cage) |
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Software | |
|
60000-FC |
ANY-maze software, FC-specific version |
|
60000 |
ANY-maze software, full license |
|
NOTE: |
ANY-maze or higher is necessary to manage Ugo Basile FC 2.1: upgrades are available |
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Components | |
|
46000-596 |
Sound Attenuating Cubicle (48.5(w)x38.5(d)x48.5(h)cm) |
|
46000-105 |
Controller on-board |
|
46000-165 |
Speaker |
|
46000-325 |
Dual (visible/I.R.) Light |
|
47400-035 |
USB Camera |